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Curcumin upregulates the expression of the oxidative stress-related proteins Nrf2 and SIRT3 in bone tissue cells. Histograms indicate the SIRT3 protein expression levels relative to the control group. Values are expressed as mean ± standard deviation for three independent experiments ( n = 3). * p < 0.05, *** p < 0.001 vs. control group, # p < 0.05, ## p < 0.01 vs. HG group.

Journal: Scientific Reports

Article Title: Curcumin preserves bone health compromised by diabetes by inhibiting osteoporosis through regulation of the SIRT3/FoxO3a signalling pathway

doi: 10.1038/s41598-025-15165-8

Figure Lengend Snippet: Curcumin upregulates the expression of the oxidative stress-related proteins Nrf2 and SIRT3 in bone tissue cells. Histograms indicate the SIRT3 protein expression levels relative to the control group. Values are expressed as mean ± standard deviation for three independent experiments ( n = 3). * p < 0.05, *** p < 0.001 vs. control group, # p < 0.05, ## p < 0.01 vs. HG group.

Article Snippet: Curcumin (Solarbio), mouse anti-SIRT3 antibody (Proteintech) , mouse anti-FoxO3a antibody (Proteintech), mouse anti-cytochrome c antibody (Proteintech), mouse anti-NRF2 antibody (Proteintech), mouse anti-NOX2 antibody (Proteintech), mouse anti-caspase 3 antibody (Proteintech), mouse anti-SOD2 antibody (Proteintech), rabbit anti-mouse SIRT3 antibody (Proteintech) Proteintech), rabbit anti-mouse Bcl2 antibody (Proteintech), mouse anti-β-actin antibody (Beijing Zhongyi Jinqiao Company), sodium pentobarbital (Sigma), paraformaldehyde (Xi’an Reagent Factory), α-D-glucose (Sigma), anti-osteocalcin (OCN) antibody (Merck & Millipore ), NQO1 (Proteintech), mouse osteoprotegerin (OPG) antibody (R&D), minimum essential medium alpha (α-MEM) (Bioind), dimethyl sulfoxide Hybri-Max (Sigma), Flow Cytometry Kit (Jiangsu Biyuntian), osteogenic induction complete medium (Pythonbio), Mitochondrial Membrane Potential Assay Kit (Jiangsu Biyuntian), ROS Reactive Oxygen Species Assay Kit (Jiangsu Biyuntian).

Techniques: Expressing, Control, Standard Deviation